F. NOGUE - TALEN and CRISPR strategies for targeted editing/tuning of the plant genome

Conventional plant breeding, which since the 1950s includes mutagenesis techniques, has been tremendously successful in securing plant production and increasing food quality for human population over history. However, it has some limitations, in particular to create new variability. In the last few years, different site directed nucleases (SDNs) allowing introducing DSBs at precise locations in the genome have been developed. They provide a very innovative way of engineering the genome by gene targeted modifications allowing gene disruption (SDN-1), gene modification (SDN-2) and gene addition (SDN-3). NHEJ-mediated repair of nuclease-induced DSBs leads to the introduction of small insertions or deletions at the targeted site, resulting in the knock-out of gene function via frame shift mutations. By co-delivering a SDN with a donor plasmid bearing locus specific homology sequences, a donor DNA can be efficiently integrated at the target locus by homologous recombination (HR). This method can be used to replace endogenous loci by superior variants or to insert single or multiple transgenes into endogenous loci. Few examples of applications of the SDNs strategies for crops genome engineering will be given during this presentation.

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Programme complet de la journée Homologous recombination in plants: TALEN and CRISPR strategies

11h-12h (in English) Seminar Room FR

• Fabien Nogué (IJPB, INRA, Versailles)
  TALEN and CRISPR strategies for targeted editing/tuning of the plant genome

14h-16h (in French) Short presentations and debate Room FR

• Servane Blanvillain-Beaufumé (UMR Résistance des Plantes aux Bioagresseurs, IRD, Montpellier)
  Creating new resistances in rice by TALEN-mediated genome editing
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• Sébastien Cunnac (UMR Résistance des Plantes aux Bioagresseurs, IRD, Montpellier)
  Utilisation du système CRISPR/Cas en mode multiplex pour l'édition du génome du riz: retour d’expérience préliminaire sur sa faisabilité

• Charles Rosenberg (LIPM, Toulouse)
  Tools for TALEN-mediated editing of the Medicago truncatula genome

• Jean-Philippe Galaud (LRSV, Toulouse)
  CRISPR-Cas9, un outil pour l'analyse fonctionnelle des Calmodulin-like protein, une famille multigénique chez Arabidopsis ?

Servane Beaufumé

Sébastien Cunnac

Contact journée : Mireille Chabaud >>>