S. Goormachtig - Integrative proteomic and metabolomic analyses place HY5 at the heart of the Arabidopsis thaliana root response to the strigolactone analog GR24

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Research interest

My research interest deals with rhizosphere interactions of plants in order to enhance growth. Emphasis has been lying on the symbiosis between legumes and rhizobia bacteria resulting in the formation of new root organs, the nodules in which the rhizobia reside and fix atmospheric nitrogen for the plant. During my PhD and early post-doc career, I was studying the interaction between the tropical legume Sesbania rostrata and the bacterium Azorhizobium caulinodans. This interaction is a model for water-tolerant nodule formation, an agricultural important trait because it can be used to supply rice with biologically fixed nitrogen. We unraveled the early signaling between the two symbiotic partners and learned about the peculiar adaptations that have evolved to enable nodulation upon submergence. We next switched to the model legume Medicago truncatula and study nodule organogenesis. Currently, we study involvement of CLE peptide signaling in nodule formation and try to get insight into the interaction of these peptide hormones with cytokinin and auxin signaling.

Apart from nodulation, we also study the signaling of an important rhizosphere signals, the strigolactones. We are interested in its effect on root architecture. We performing a combined proteome, transcriptome and genetic approach in order to elucidate the signaling components acting downstream of strigolactones to influence root architecture.

Currently we also have started to unravel the molecular basis of growth promoting by various plant growth promoting bacteria. Our goal is to elucidate the molecular networks that are activated by various growth promoting bacteria in order to use this knowledge for direct application of probiotics for crop growth.

Important articles of the last five years

Articles published in journals listed in the ISI Web of Knowledge

1)      9.293       5/173        Capoen, W., Den Herder, J., Sun, J., Verplancke, C., De Keyser, A., De Rycke, R., Goormachtig, S., Oldroyd, G., and Holsters, M. (2009). Calcium spiking patterns and the role of the calcium/calmodulin-dependent kinase CCaMK in lateral root base nodulation of Sesbania rostrata. Plant Cell 21, 1526-1540.

2)      6.451       8/188        Mortier, V., Den Herder, G., Whitford, R., Van de Velde, W., Rombauts, S., D'haeseleer, K., Holsters, M., and Goormachtig, S. (2010). CLE peptides control Medicago truncatula nodulation locally and systemically. Plant Physiol. 153, 222-237

3)      11.808    2/195        De Geyter*, N., Gholami*, A., Goormachtig, S., and Goossens, A. (2012). Transcriptional machineries in jasmonate-elicited plant secondary metabolism. Trends Plant Sci. 17, 349-359. (* equal contribution)

4)      6.582       7/195        Mortier, V., De Wever, E., Vuylsteke, M., Holsters, M., and Goormachtig, S. (2012). Nodule numbers are governed by interaction between CLE peptides and cytokinin signaling. Plant J. 70, 367-376

5)      6.555       8/190        Osipova, M.A., Mortier, V., Demchenko, K.N., Tsyganov, V.E., Tikhonovich, I.A., Lutova, L.A., Dolgikh, E.A., and Goormachtig, S. (2012). WUSCHEL-RELATED HOMEOBOX5 gene expression and interaction of CLE peptides with components of the systemic control add two pieces to the puzzle of autoregulation of nodulation. Plant Physiol. 158, 1329-1341.

6)      6.555       8/195        Rasmussen, A., Mason, M.G., De Cuyper, C., Brewer, P.B., Herold, S., Agusti, J., Geelen, D., Greb, T., Goormachtig, S., Beeckman, T., and Beveridge, C.A. (2012). Strigolactones suppress adventitious rooting in Arabidopsis and pea. Plant Physiol. 158, 1976-1987.

7)      6.555       8/195        Mayzlish-Gati, E., De Cuyper, C., Goormachtig, S., Beeckman, T., Vuylsteke, M., Brewer, P.B., Beveridge, C.A., Yermiyahu, U., Kaplan, Y., Enzer, Y., Wininger, S., Resnick, N., Cohen, M., Kapulnik, Y., and Koltai, H. (2012). Strigolactones are involved in root response to low phosphate conditions in Arabidopsis. Plant Physiol. 160, 1329-1341.

8)      5.135       14/195     Mortier, V., Holsters, M., and Goormachtig, S. (2012). Never too many? How legumes control nodule numbers. Plant Cell Environ. 35, 245-258.

9)      38.597    1/56          Pollier, J., Moses, T., González-Guzmán, M., De Geyter, N., Lippens, S., Vanden Bossche, R., Marhavý, P., Kremer, A., Morreel, K., Guérin, C.J., Tava, A., Oleszek, W., Thevelein, J.M., Campos, N., Goormachtig, S., and Goossens, A. (2013). The protein quality control system manages plant defense compound synthesis. Nature 504, 148-152.

10)    6.126       10/197     Rasmussen, A., Heugebaert, T., Matthys, C., Van Deun, R., Boyer, F.-D., Goormachtig, S., Stevens, C., and Geelen, D. (2013). A fluorescent alternative to the synthetic strigolactone GR24. Mol. Plant 6? 100-112.

11)    3.347       28/197     Rasmussen, A., Depuydt, S., Goormachtig, S., and Geelen, D. (2013). Strigolactones fine-tune the root system. Planta 238, 615-626.Shared last author.

12)    10.178    1/59          Gholami, A., De Geyter, N., Pollier, J., Goormachtig, S., and Goossens, A. (2014). Natural product biosynthesis in Medicago species. Nat. Prod. Rep., 31(3):356-80

13)    6.736       6/197        Mortier, V., Wasson, A., Jaworek, P., De Keyser, A., Decroos, M., Holsters, M., Tarkowski, P., Mathesius, U., and Goormachtig, S. (2014). Role of LONELY GUY genes in indeterminate nodulation on Medicago truncatula. New Phytol. 202 (2): 582-93.

14)    6.126       10/197     Boyer, F.-D., de Saint Germain, A., Pouvreau, J.-B., Clavé, G., Pillot, J.-P., Roux, A., Rasmussen, A., Depuydt, S., Lauressergues, D., Frei dit Frey, N., Heugebaert, T.S.A., Stevens, C.V., Geelen, D., Goormachtig, S., and Rameau, C. (2014). New strigolactone analogues as plant hormones with low activities in the rhizosphere. Mol. Plant, 7(4):675-90

15)    5.135       14/197     Marquez-Garcia, B., Njo, M., Beeckman, T., Goormachtig, S., and Foyer, C.H. (2014). A new role for glutathione in the regulation 1 of root architecture linked to strigolactones. Plant Cell Environ. 37, 488-498.

16)    1.706       53/75        Pertry, I., Sabbadini, S., Goormachtig, S., Lokko, Y., Gheysen, G., Burssens, S., and Mezzetti, B.. (2014). Biosafety capacity building: experiences and challenges from a distance learning approach. New Biotechnol. 31, 64-68.

17)    9.937       3/45          Motte, H., Vercauteren, A., Depuydt, S., Landschoot, S., Geelen, D., Werbrouck, S., Goormachtig, S., Vuylsteke, M., and Vereecke, D. (2014). Combining linkage and association mapping identifies RECEPTOR-LIKE PROTEIN KINASE1 as an essential Arabidopsis shoot regeneration gene. PNAS, in press