Dr. Herman Hofte

H. HÖFTE - Plant cell wall patterning through protein-peptide-pectin interaction

FR AIB
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H. HÖFTE - Plant cell wall patterning through protein-peptide-pectin interaction

H. HÖFTE - Plant cell wall patterning through protein-peptide-pectin interaction

12 mai 2023

Salle de séminaire FR AIB

Herman HÖFTE, chef de l'équipe "Paroi primaire" au sein de l'institut Jean-Pierre Bourgin et spécialiste de la signalisation de la paroi cellulaire, viendra présenter ses travaux le 12 mai prochain prochain à 11h en salle de séminaire FR.

Abstract

Cell wall assembly and expansion in root hairs: pRALF22:GFP in Arabidopsis root.

Cell wall assembly and expansion in root hairs: pRALF22:GFP in Arabidopsis root

RALF22 is a pectin-binding peptide, expressed in trichoblasts during root hair elon-

gation, with a dual structural and signaling role in cell wall assembly and expansion.

Photo: Sebastjen Schoenaers

Pressurized cells with strong walls make up the hydrostatic skeleton of plant organs. Assembly and expansion of such a stressed wall depends on a family of secreted peptides (RALFs) which, curiously, bind both a membrane receptor complex and cell wall proteins (LRXs) in a mutually exclusive way. Here we show in root hairs that the polycationic peptide RALF22 have a dual structural and signaling role in cell expansion. In root hairs, together with LRX1/2 proteins, RALF22 directs the compaction of newly deposited polyanionic pectin polymers into periodic circumferential rings at the tip. If not bound to LRX1/2, RALF22 induces the formation of a ternary LLG1-RALF22-FER complex which triggers adaptive cellular responses. These findings show how a peptide simultaneously functions as a structural component organizing cell wall architecture and as a signaling molecule that regulates this process. A similar process controls the assembly of a dendritic pattern in pollen tube cell walls. Similar mechanisms may underlie wall assembly and growth of other cell types.

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H.Hofte

Dr Herman Höfte received his PhD degree at the University of Ghent (Belgium) in 1988 on the study of the insecticidal crystal proteins from Bacillus thuringiensis and the generation of the first insect-resistant transgenic plants. His thesis supervisor was Marc Van Montagu and his work was carried out in the, at that time, start-up biotechnology company Plant Genetic Systems. He then moved to the laboratory of Maarten Chrispeels at the University of California, San Diego in 1989, where he worked as a post-doctoral fellow on the intracellular targeting of vacuolar membrane proteins in plants using aquaporins as a model system. In 1992 he took up a position as a research scientist at the National Institute of Agronomic Research (INRA) in Versailles, France where he is studying the synthesis and assembly of cell walls using multidisciplinary approaches, including genetics, live cell imaging, biochemistry and biophysics. He is in particular interested in the synthesis of cellulose and pectin, cell wall integrity signaling and the role of the cell wall in growth control and in responses to the environment. He has also coordinated a large French “Stimulus Initiative” project “Biomass For the Future” aiming at developing new dedicated biomass grasses and uses thereof.

 

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Date de modification : 07 juillet 2023 | Date de création : 06 juin 2023