NanoTemper2

NanoTemper

NanoTemper

Microscale Thermophoresis analysis enables to study biomolecular interactions without prior purification or immobilization of the interactors. It gives access to the determination of the binding properties in terms of affinity (in the range from 10-2 to 10-11 M) and stoichiometry. The principle of the method takes into account the motion of molecules along microscopic temperature gradients and detects changes depending on their free or bound states. These changes are detected and quantified by measuring changes in fluorescence associated to one of the interactors.

In January 2016, the FR-AIB has acquired a Monolith NT.115 (Green/Red) from NanoTemper (http://www.nanotemper-technologies.com) with the financial support of the IDEX (GO-AHEAD project coordinated by A. Jauneau), INRA and the PICT platform (IPBS, Toulouse). The instrument is compatible with the use of fluorophores with excitation wavelengths in the range 514-652 nm.

NanoTemper1
NanoTemper2

Contacts

Instrument disponibilities

https://calendar.google.com/calendar/embed?src=nanotemper.tlse%40gmail.com&ctz=Europe/Paris

2016/01/21 - The instrument installation, room D201, LIPM

Program of the day

  • 09:30 – 10:15 : seminar on thermophoresis (seminar room FR AIB)
  • 10:15 – 12:00 : samples labeling, standards measurements and demo
  • 12:00 – 13:00 : lunch
  • 13:00 – XX:XX : samples measurement and access to the instrument

Modification date: 27 June 2023 | Publication date: 14 January 2016 | By: JJB, JC