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The FR AIB is closely associated with the Toulouse Proteomics platform, whose main site is at the Institute of Pharmacology and Structural Biology (IPBS). This platform uses mass spectrometry (MS) for large scale protein analyses ranging from initial identification to detailed chemical analysis and studies of molecular interactions. A FR AIB staff member (C. Pichereaux), specialist in mass spectrometry, is in charge of the various FR proteomics projects.

Services provided by the IPBS proteomics unit:

  • Protein identification
  • Post-translational modification studies
  • Analysis of protein complexes and the identification of interacting partners
  • Quantitative global analysis of proteomes
  • Targeted analysis of proteins of interest
  • Bioinformatics analysis of MS data

Specialized equipment:

  • Several latest generation mass spectrometers comprise the principal equipment of the platform:
    • nanoLC-ESI MS/MS using high resolution and high acquisition speed machines such as Orbitrap (thermo Scientific) with several possible fragmentation modes (CIS, HCD, ETD) for global proteomics analysis
    • Q-TRAP mass spectrometry (Applied Biosystems) dedicated to targeted proteomics analysis
    • MALDI-TOF mass spectrometry (Applied Biosystems) 
  • Protein-protein interaction studies can be performed with the Biacore system.